Journal: Frontiers in Microbiology

Article Title: GRB7-mediated enhancement of cell malignant characteristics induced by Helicobacter pylori infection

doi: 10.3389/fmicb.2024.1469953

Figure Lengend Snippet: Helicobacter pylori increases intracellular GRB7 expression. (A,B) Representative IHC staining images of GRB7 in GC tissues and their paired normal gastric mucosal tissues ( n = 10). Scale bar, 200 μm. (B) Quantification of (A) . (C) Expression levels of GRB7 in different cell lines. (D,E) Western blot (D) and RT-qPCR (E) analysis of the expression of GRB7 in cells after H. pylori infection. (F,G) Representative IHC images of GRB7 expressed in H. pylori -positive ( n = 12) or -negative gastritis tissues ( n = 15). Scale bar, 200 μm. (G) Quantification of (F) . All experiments were conducted in triplicates and the data shown are represented as mean ± SD. Data were analyzed using an independent samples t -test. ** p < 0.01 and *** p < 0.001.

Article Snippet: IHC staining was performed according to the usual procedure by using IHC test reagents (ZSGB-BIO, Beijing, China) ( ).

Techniques: Expressing, Immunohistochemistry, Western Blot, Quantitative RT-PCR, Infection

Journal: Frontiers in Microbiology

Article Title: GRB7-mediated enhancement of cell malignant characteristics induced by Helicobacter pylori infection

doi: 10.3389/fmicb.2024.1469953

Figure Lengend Snippet: Confirmation of GRB7 roles in H. pylori pathogenesis in vivo . (A,B) Rapid urease test (A) and Giemsa staining (B) confirming H. pylori colonization. Black arrows indicate curved H. pylori (400×). (C) HE staining of mice gastric epithelium. Red arrows indicate rounded vacuolated cup-shaped cells, black arrows show inflammatory cells ( n = 3). Bar in upper image indicates 100 μm, and in lower image indicates 50 μm. (D) The amount of IL6 in serum. (E) Expression of GRB7, p-ERK, and p-STAT3 in gastric tissues of mice. (F,G) Expression of E-cadherin and Ki67 in gastric mucosa detected by IHC ( n = 5). Scale bar, 100 μm. All experiments were conducted in triplicates and the data shown are represented as mean ± SD. Data were analyzed using an independent samples t -test. Control, brain heart infusion broth treated mice; H. pylori , H. pylori -infected mice. ** p < 0.01, and *** p < 0.001.

Article Snippet: IHC staining was performed according to the usual procedure by using IHC test reagents (ZSGB-BIO, Beijing, China) ( ).

Techniques: In Vivo, Staining, Expressing, Control, Infection

Journal: Blood Advances

Article Title: Acute myeloid leukemia–induced remodeling of the human bone marrow niche predicts clinical outcome

doi: 10.1182/bloodadvances.2020001808

Figure Lengend Snippet: AML infiltration induces proliferation of bone marrow MSPCs. (A) Exemplary procedure for quantification of CD271. First, tile images were fused into larger images (1). Second, a color deconvolution was performed (ImageJ, IHC Profiler plugin) to obtain separate hematoxylin and DAB images. The hematoxylin image was thresholded to obtain a mask of the total bone region (2). The DAB image (3) was inverted and thresholded to obtain a mask of the positive DAB regions (4). Scale bars, 1 mm. (B) Representative images of bone marrow biopsy samples of non-leukemic donors (controls) and AML patients stained for CD271+ MSPCs (brown) and counterstained with hematoxylin (blue). Scale bars, 400 µm. (C) Quantification of CD271+ bone marrow MSPCs in controls (n = 58) and AML patients (n = 36); CD271+ area is calculated by CD271+ stained area divided by total tissue surface. (D) Left: representative image from bone marrow after silver staining representing reticular fibers. Right: image converted by trainable Weka segmentation showing 3 different classes: red, reticular fiber; green, tissue; purple, background. Scale bars, 50 µm. (E) Representative images of bone marrow biopsy samples from controls and AML patients stained for reticular fibers with silver staining (black fiber) and counterstained with the fast-red nuclear solution. Scale bars, 100 µm. (F) Quantification of reticular fibers in control (n = 19) and AML (n = 37) bone marrow by calculating positive silver stained area divided by total tissue surface (n = 56). Data are shown as mean ± standard error of the mean (SEM). *P < .05; **P < .01 (determined by Mann-Whitney U test).

Article Snippet: Color deconvolution of the images was performed to obtain separate hematoxylin and DAB images using the ImageJ plugin IHC Profiler ( https://sourceforge.net/projects/ihcprofiler/ ).

Techniques: Staining, Silver Staining, Control, MANN-WHITNEY